Not really, I've seen the movie posters and heard people talk about them, but I don't know anything about them except Thor is the Norse god of thunder and Captain America has a shield and is really... patriotic? Also I didn't recognize any of the villains except Green Goblin and Doc Oc, since they were in the first 2 Spiderman movies. I don't think there were any supervillians in the Ironman, Hulk, or Wolverine movies.
I guess the main thing was that I didn't understand the story or the motivation behind the characters - it seems like heroes didn't always fight their arch-nemesis supervillian, they were just kind of introduced and taken away from the story in a seemingly random manner - I remember the Juggernaut bossfight, right before you fight him you lose one of the characters that you did 90% of the level with, and then are introduced to Beast (who the

?) and he isn't even that vital in the fight, you use him like once or twice and it left me thinking, why? Why did they drop him in right before the boss fight, only to play a very minor role? Was he important story wise? I couldn't tell, and honestly, after 10 levels I still couldn't tell what the story was other than "supervillians are

ing shit up, superheroes to the rescue!"